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We have successfully purified the antibodies for COVID19 serological tests by Affinity Chromatography using Protein G Agarose Resin 4 Rapid Run™ from Agarose Bead technologies.
The chromatography resin ensured good selectivity and capacity and we obtained highly pure and functional antibodies.
Span Diagnostics recommends the products from Agarose Bead Technologies!
“We were trying to purify recombinant SARS-CoV-2 antigens, but we were at the bottleneck of our experiments and we realized that new tools were needed. ABT has offered us a the solution, a chelate resin that has been the key to optimizing the purification of the virus proteins that have become part of some serological diagnostic kits. The purification process has been shortened by 2 days and the protein we obtain is completely native, without previously going through any denaturation cycle – this is very important”. Time and quality are two critical factors for the generation of ELISA diagnostic tests.
Department of Micro and Nanotechnology Technical University of Denmark, DENMARK
"In our research within micro-biotechnology we often have a need for customized solutions that suits our technology platform. Agarose Bead Technologies has provided us with excellent customized beads in a short time, which was essential for the successfulness of our research project. Thus, we have no problems recommending ABT when it comes to product quality and delivery."
Department of Molecular Microbiology·Centro de Investigaciones Biológicas (CIB-CSIC) Madrid, SPAIN
In my lab at CIB-CSIC, we have been using (since 1996) Ni2+-IMAC for the purification of many different His-tagged proteins at large scales (10-100 mg). We are very demanding in terms of the yields and the final purity grade of our protein preps, because both of them are key factors in the structural and biochemical characterization of proteins, our main research interest. One of the major recent improvements in our daily work has been the discovery of the high-density IDA-agarose beads developed and manufactured by ABT: They match (and in some occasions, even outperform) the results previously achieved with other commercial metal-chelating supports, specially when packing of relatively large volumes (> 5 ml) of gel are required and there is a need for a mechanically robust and reliable, yet affordable, chromatographic material. Thus ABT beads have been included as essential resources in all our recent scientific achievements.
Rensselaer Polytecnnic Institute, Deparment of Chemical and Biological Engineering, USA
“In our sulfotransferase immobilization experiments, enzyme immobilized on ABT’s Glyoxal Agarose beads showed highest activity and stability in comparing with other beads.” Research projects in biochemical engineering emphasize biocatalysis, bioseparations,and metabolic engineering. Fundamental and applied aspects of enzyme technology,mammalian cell culture, membrane sorption and separation, displacement chromatography,and salt-induced precipitation are important areas of focus.
Centro de Investigaciones Biológicas-CSIC, Department of Molecular Microbiology, Madrid, SPAIN
"Several companies offer Nickel activated chelating beads; however, Agarose Bead Technologies (ABT) has taken a different approach and offers a comprehensive range of beads with different chelating metals (nickel, cobalt, copper, zinc) so that a much wider range of proteins can be effectively separated. In addition, the chelates are offered at different loading capacities (Low, High, Very High) so that the beads can deal effectively with technical issues such as different sizes of proteins, recovery yields and specificity. ABT offers test kits with a range of chelates in small quantities so that you can optimize your separation for a very low price. The chelates have been very widely accepted by research labs and we have several testimonials available".
Institut für Biologie Berlin, Humboldt- Universität Zu Berlin, GERMANY
“We are studying the function and mechanisms of cofactor-containing enzymes. The focus is on the function of complex metal clusters and flavins in redox catalysis. Therefore we need a lot of purified proteins from various organisms. The empty FPLC Columns from ABT are very helpful for our working with different chromatographic material. The handling is good and also the quality and price."

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