Institute of Biotechnology, University of Manchester, UK
“The advantage of using ABTs Zn2+ resin in routine protein purifications is the requirement for fewer wash steps, providing the possibility for significant time and cost savings” In the purification of a protein, the performance of the resin in terms of duration of the overall process/number of steps required is just as important as the purity of the resulting protein. For clients using our products on a larger scale, the number of process steps therefore becomes an important economic consideration".
University of Groningen, Department of Chemical Engineering, NETHERLANDS
“Agarose Bead Technologies facilitates this screening by offering their range in immobilization beads in a convenient test kit. This makes selection of the best carrier possible without the need to buy relatively large (and expensive) amounts of resins.” Sometimes the only way to know which purification or immobilization resin is the best for each work is to test with different degrees of activations. ABT offers inexpensive test kits for prior practical evaluation of the resin that will give best results in your process.
Centro de Investigaciones Biológicas-CSIC, Department of Molecular Microbiology, Madrid, SPAIN
"Several companies offer Nickel activated chelating beads; however, Agarose Bead Technologies (ABT) has taken a different approach and offers a comprehensive range of beads with different chelating metals (nickel, cobalt, copper, zinc) so that a much wider range of proteins can be effectively separated. In addition, the chelates are offered at different loading capacities (Low, High, Very High) so that the beads can deal effectively with technical issues such as different sizes of proteins, recovery yields and specificity. ABT offers test kits with a range of chelates in small quantities so that you can optimize your separation for a very low price. The chelates have been very widely accepted by research labs and we have several testimonials available".
Rensselaer Polytecnnic Institute, Deparment of Chemical and Biological Engineering, USA
“In our sulfotransferase immobilization experiments, enzyme immobilized on ABT’s Glyoxal Agarose beads showed highest activity and stability in comparing with other beads.” Research projects in biochemical engineering emphasize biocatalysis, bioseparations,and metabolic engineering. Fundamental and applied aspects of enzyme technology,mammalian cell culture, membrane sorption and separation, displacement chromatography,and salt-induced precipitation are important areas of focus.
Department of Molecular Microbiology·Centro de Investigaciones Biológicas (CIB-CSIC) Madrid, SPAIN
In my lab at CIB-CSIC, we have been using (since 1996) Ni2+-IMAC for the purification of many different His-tagged proteins at large scales (10-100 mg). We are very demanding in terms of the yields and the final purity grade of our protein preps, because both of them are key factors in the structural and biochemical characterization of proteins, our main research interest. One of the major recent improvements in our daily work has been the discovery of the high-density IDA-agarose beads developed and manufactured by ABT: They match (and in some occasions, even outperform) the results previously achieved with other commercial metal-chelating supports, specially when packing of relatively large volumes (> 5 ml) of gel are required and there is a need for a mechanically robust and reliable, yet affordable, chromatographic material. Thus ABT beads have been included as essential resources in all our recent scientific achievements.
University of Cambridge Dept of Chemistry, UK
"We have since purchased 500 ml of Agarose Bead Technologies resin and have used this to purify several proteins in our lab. We have purified all-alpha helical and all-beta sheet proteins, from 100 - 300 amino acids in size, expressed in E.coli. We find that the ABT resin performs as well as that of other leading manufacturers but costs less. This has allowed us to make a significant saving as we work on many mutant proteins and therefore use a lot of pre-charged resin".

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