Sepadextran™-50 Medium

Sepadextran™-50 Medium

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Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-50 can be used for protein and nucleic acid purifications being the exclusion limit 25 kD for proteins and 20 bases for nucleic acids.

Medium grade is suitable for separations at high flow rates and low operating pressures.
Product Name Sepadextran™ 50 Medium
Cat. No. SD50M-X
Matrix Crosslinked dextran
Water Regain 4.80-5.20 ml/g
Swelling 9-11 ml/g
Dry Particle Size 50-150 µm (>80%)
Wet Particle Size 100-300 µm
Maximum Operating Pressure Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=145
Fractionation Range 1.5-30 kD for globular proteins, 0.5-10 kD for dextrans
pH Stability 2.0 to 13
Autoclavable 121˚C, pH 7.0 (30 minutes)
Chemical Stability All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile
Storage Temperature Ambient
Technical Specifications View
Procedure of Use View