Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-25 can be used for protein and nucleic acid purifications being the exclusion limit 5 kD for proteins and 10 bases for nucleic acids. Desalting (before IEX and after HIC or Affinity Chromatography) & buffer exchange (between different chromatography steps) are other uses.
Medium grade is suitable for separations at high flow rates and low operating pressures.
Medium grade is suitable for separations at high flow rates and low operating pressures.
| Product Name | Sepadextran™-25 Medium |
| Cat. No. | SD25M-X |
| Matrix | Crosslinked dextran |
| Water Regain | 2.15-2.25 ml/g |
| Swelling | 4-6 ml/g |
| Dry Particle Size | 50-150 µm (>80%) |
| Wet Particle Size | 85-260 µm |
| Maximum Operating Pressure | Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=80 |
| Fractionation Range | 1.5 kD for globular proteins, 100-5,000 D for dextrans |
| pH Stability | 2.0 to 13 |
| Autoclavable | 121˚C, pH 7.0 (30 minutes) |
| Chemical Stability | All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile |
| Storage Temperature | Ambient |
