Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins. Nickel Rapid Run™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially designed for large scale His-tagged protein purifications.
|Product Name||Nickel Rapid Run™|
|Bead Geometry & Size||Spherical,Standard ~ 50-150 µm|
|Maximum Flow Rate at 15 cm Bed Height||≥ 1000 cm/h|
|Maximum Pressure at 15 cm bed Height||≥ 300 kPa|
|Exclusion Limit Da||~4×10^6|
|Loading Capacity (μmol me2+/ml gel)||~20|
|Antimicrobial Agent||20% Ethanol|