Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins.
Nickel Chelate Kit includes: 2ml of High Density Nickel & 2ml of Low Density Nickel.
High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel has a lower amount of groups and usually improves the selectivity/specificity.
| Product Name | Nickle Chelate Kit |
| Cat. No. | 6BCL-KNi-2 |
| Bead Geometry & Size | Spherical,Standard: 50-150 µm |
| Description | Recommended for proteins that are easy to separate |
| Crosslinked | Yes |
| Agarose % | 6% |
| Matrix | Stable in all commonly used reagents |
| Contains | 2ml High Density Nickel & 2ml Low Density Nickel |
| Binding/Loading Capacity (μmol Me2+/ml gel) | 20-40(High Density) & 5-19 (Low Density) |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-8˚C |
