Still not sure which method to use when it comes to ligand immobilization?

Agarose-based beads are highly porous, mechanically resistant, chemically and physically inert, and hydrophilic. These properties improved by means of a cross-linking step render agarose beads ideal for conjugation of ligands.

The activation of the resin and the coupling chemistry of biologic or synthetic ligands are important factors in the development of successful affinity chromatography methods, for the isolation of the target molecule.

The use of Cyanogen Bromide (CNBr) activated matrices is a well-known, quick and familiar technique for the coupling of ligands to generate affinity chromatography resins. Coupling is via primary amino groups for both Glyoxal and CNBr agarose. Historically, Cyanogen Bromide (CNBr) activated matrices have been very popular. But this type of matrix has some key disadvantages such as ligand leaching and ionic properties.

Comparatively, the use of proteins immobilized on Glyoxal agarose has been proven to be very advantageous due to increased stabilization of the adsorbent protein, while the inert surface prevents nonspecific binding of other biomacromolecules.

Likewise, there are many advantages that Glyoxal resins provide compared to Cyanogen Bromide. When we compare the properties of both products (Table 1), it is clear why Glyoxal is the best option.  

Glyoxal

Table 1. Comparison between CNBr agarose and Glyoxal agarose properties.

Furthermore, Glyoxal agarose presents many other properties which are very suitable for achieving a very intense multipoint covalent attachment of the protein. The following table (Table 2)  highlight some of the characteristics of Glyoxal Agarose, which make it an optimal and reliable product.

glyoxal

Table 2. Characteristics comparison between CNBr agarose and Glyoxal agarose properties.

Glyoxal Agarose, the perfect resin for conjugation.

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