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High Density Nickel

High Density Nickel

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CAT. No.


Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins.
High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. The resin is adequate to work in batch or column purifications (Low Flow).


  • Lechner, Horst et al. “Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations.” Biotechnology Journal 13.3 (2018): n. pag. Biotechnology Journal. 
  • Marsden, A. P. et al. Investigating the Effect of Chain Connectivity on the Folding of a Beta-Sheet Protein On and Off the Ribosome. Journal of Molecular Biology 430, 5207–5216 (2018).
  • Sanchez-Rodriguez, Ana et al. “Recombinant Rabbit Beta Nerve Growth Factor Production and Its Biological Effects on Sperm and Ovulation in Rabbits.” PLoS ONE 14.7 (2019): n. pag. PLoS ONE. 
Product NameHigh Density Nickel
Cat. No.6BCL-QHNi-X
Bead Geometry & SizeSpherical Standard: ~50-150 μm
Crosslinked Yes
Agarose % 6%
MatrixStable in all commonly used reagents
Binding/Loading Capacity (μmol Me2+/ml gel)20-40
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-8ºC
Technical Specifications View
Procedure of Use View