Nickel & Cobalt Chelate Kit

Nickel & Cobalt Chelate Kit

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This kit is recommended because Nickel and Cobalt resins capture the majority of the easy to separate histidine-tagged proteins. Nickel chelates recognize two exposed histidine tags. Cobalt chelates recognize such groups if they are in vicinal (neighboring) positions. Nickel & Cobalt Chelate Kit contains 2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel & High Density Cobalt usually improve the selectivity.
Product Name Nickel & Cobalt Chelate Kit
Cat. No. 6BCL-KNiCo-2
Bead Geometry & Size Spherical,Standard: 50-150 µm
Description Recommended for proteins that are easy to separate
Crosslinked Yes
Agarose % 6%
Matrix Stable in all commonly used reagents
Contains 2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt
Binding/Loading Capacity (μmol Me2+/ml gel) 20-40(High Density) & 5-19 (Low Density)
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-8˚C
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