This kit is recommended because Nickel and Cobalt resins capture the majority of the easy to separate histidine-tagged proteins. Nickel chelates recognize two exposed histidine tags. Cobalt chelates recognize such groups if they are in vicinal (neighboring) positions. Nickel & Cobalt Chelate Kit contains 2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel & High Density Cobalt usually improve the selectivity.
|Product Name||Nickel & Cobalt Chelate Kit|
|Bead Geometry & Size||Spherical,Standard: 50-150 µm|
|Description||Recommended for proteins that are easy to separate|
|Matrix||Stable in all commonly used reagents|
|Contains||2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt|
|Binding/Loading Capacity (μmol Me2+/ml gel)||20-40(High Density) & 5-19 (Low Density)|
|Antimicrobial Agent||20% Ethanol|