Nickel & Cobalt Chelate Kit+ 30 empty mini columns

Nickel & Cobalt Chelate Kit+ 30 empty mini columns

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This kit is recommended because Nickel and Cobalt resins capture the majority of the easy to separate histidine-tagged proteins. Nickel chelates recognize two exposed histidine tags. Cobalt chelates recognize such groups if they are in vicinal (neighboring) positions. Nickel and Cobalt Chelate Kit contains 2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt, and 30 empty mini-columns to allow for centrifugation steps during the procedure. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel & High Density Cobalt usually improve the selectivity/specificity.
Product Name Nickel & Cobalt Chelate Kit+ 30 empty mini columns
Cat. No. 6BCL-KNiCoMC-2
Bead Geometry & Size Spherical,Standard: 50-150 µm
Description Recommended for proteins that are easy to separate
Crosslinked Yes
Agarose % 6%
Matrix Stable in all commonly used reagents
Contains 2ml of High Density Nickel, 2ml of Low Density Nickel & 2ml of High Density Cobalt, and 30 empty mini-columns
Binding/Loading Capacity (μmol Me2+/ml gel) 20-40(High Density) & 5-19 (Low Density)
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-8˚C
Technical Specifications View
Procedure of Use View