Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate His-tagged proteins. Nickel Chelate Kit includes: 2ml of High Density Nickel & 2ml of Low Density Nickel. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel has a lower amount of groups and usually improves the selectivity/specificity.
|Product Name||Nickle Chelate Kit|
|Bead Geometry & Size||Spherical,Standard: 50-150 µm|
|Description||Recommended for proteins that are easy to separate|
|Matrix||Stable in all commonly used reagents|
|Contains||2ml High Density Nickel & 2ml Low Density Nickel|
|Binding/Loading Capacity (μmol Me2+/ml gel)||20-40(High Density) & 5-19 (Low Density)|
|Antimicrobial Agent||20% Ethanol|