Nickel Chelate Kit + 20 empty mini columns

Nickel Chelate Kit + 20 empty mini columns

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Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate proteins. This Kit includes: 2ml of High Density Nickel, 2ml of Low Density Nickel and 20 empty mini-columns. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel has a lower amount of groups and it usually improves the selectivity/specificity in the purification procedure. This kit also contains mini columns to allow for centrifugation steps during the procedure.
Product Name Nickel Chelate Kit + 20 empty mini columns
Cat. No. 6BCL-KNiMC-2
Bead Geometry & Size Spherical,Standard: 50-150 µm
Description Recommended for proteins that are easy to separate
Crosslinked Yes
Agarose % 6%
Matrix Stable in all commonly used reagents
Contains 2ml of High Density Nickel, 2ml of Low Density Nickel and 20 empty mini-columns
Binding/Loading Capacity (μmol Me2+/ml gel) 20-40(High Density) & 5-19 (Low Density)
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-8˚C
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Procedure of Use View