Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases- this resin works well with relatively easy to separate proteins. This Kit includes: 2ml of High Density Nickel, 2ml of Low Density Nickel and 20 empty mini-columns. High Density Nickel provides a higher amount of available groups and usually allows for higher binding capacity. Low Density Nickel has a lower amount of groups and it usually improves the selectivity/specificity in the purification procedure. This kit also contains mini columns to allow for centrifugation steps during the procedure.
|Product Name||Nickel Chelate Kit + 20 empty mini columns|
|Bead Geometry & Size||Spherical,Standard: 50-150 µm|
|Description||Recommended for proteins that are easy to separate|
|Matrix||Stable in all commonly used reagents|
|Contains||2ml of High Density Nickel, 2ml of Low Density Nickel and 20 empty mini-columns|
|Binding/Loading Capacity (μmol Me2+/ml gel)||20-40(High Density) & 5-19 (Low Density)|
|Antimicrobial Agent||20% Ethanol|