Nickel NTA Agarose Resin

Nickel NTA Agarose Resin

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This resin consists of crosslinked agarose derivatized with Nitrilotriacetic acid (NTA) and provides good properties working in native or denaturing conditions. This resin can recover His-tagged proteins from a variety of expression systems such as baculovirus, yeast, mammalian and bacterial cells.

REFERENCES:

  • Deneka, Dawid et al. “Structure of a Volume-Regulated Anion Channel of the LRRC8 Family.” Nature 558.7709 (2018): 254–259. Nature.
  • Petrenčáková, Martina et al. “Conformational Properties of LOV2 Domain and Its C450A Variant within Broad PH Region.” Biophysical Chemistry 259 (2020): n. pag. Biophysical Chemistry.
  • Qian, Yong et al. “A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator.” Nature Methods 16.2 (2019): 171–174. Nature Methods. 
  • Dunne, Matthew, and Martin J. Loessner. “Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria.” Methods in Molecular Biology. Vol. 1918. Humana Press Inc., 2019. 67–86. Methods in Molecular Biology. 
  • Huo, Jiangdong et al. “Structural Characterisation of a Nanobody Derived from a Naïve Library That Neutralises SARS-CoV-2.” (2020): n. pag. Web.

Product NameNickel NTA Agarose Resin
Cat. No.6BCL-NTANi-X
Bead Geometry & SizeSpherical,Standard: ~ 50-150 µm
Crosslinked Yes
Agarose % 6%
LigandNitrilotriacetic Acid (NTA)
Static Binding Capacity ≥ 50 mg/ ml gel
Antimicrobial Agent 15 % Ethanol of total volume
Storage Temperature 4-8˚C
Technical Specifications View
Procedure of Use View