Nickel Rapid Run™

Nickel Rapid Run™

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Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins. Nickel Rapid Run™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially designed for large scale His-tagged protein purifications.

Product Name Nickel Rapid Run™
Cat. No. 6NiRR-X
Bead Geometry & Size Spherical,Standard ~ 50-150 µm
Crosslinked Highly crosslinked
Agarose % 6%
Maximum Flow Rate at 15 cm Bed Height ≥ 1000 cm/h
Maximum Pressure at 15 cm bed Height ≥ 300 kPa
Exclusion Limit Da ~4×10^6
Ligand Iminodiacetic Acid
Loading Capacity (μmol me2+/ml gel) ~20
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-8˚C
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Procedure of Use View