6% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. It is a gel filtration resin commonly used for coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.
REFERENCES:
- Benítez-Mateos, Ana I. et al. “Expanding One-Pot Cell-Free Protein Synthesis and Immobilization for On-Demand Manufacturing of Biomaterials.” ACS Synthetic Biology 7.3 (2018): 875–884. ACS Synthetic Biology.
- Yilmaz, Gulden, Esther E. Biswas-Fiss, and Subhasis B. Biswas. “Genetic Variations in the DNA Replication Origins of Human Papillomavirus Family Correlate with Their Oncogenic Potential.” Biochimica et Biophysica Acta - General Subjects 1862.4 (2018): 979–990. Biochimica et Biophysica Acta - General Subjects.
- Ruzic, Lucija, Juan M. Bolivar, and Bernd Nidetzky. “Glycosynthase Reaction Meets the Flow: Continuous Synthesis of Lacto-N-Triose II by Engineered β-Hexosaminidase Immobilized on Solid Support.” Biotechnology and Bioengineering 117.5 (2020): 1597–1602. Biotechnology and Bioengineering.
- Claudia Ubilla et al. "Synthesis of Lactulose in Continuous Stirred Tank Reactor With β-Galactosidase of Apergillus oryzae Immobilized in Monofunctional Glyoxyl Agarose Support" Biotechnology and Bioingenieering. Volume 8, 669 (2020).
| Product Name | 6% BCL Agarose Bead Standard (50-150µm) |
| Cat. No. | A-1061S-X |
| Bead Geometry & Size | Spherical ~50-150 µm |
| Bead Mean Diameter d50v | ~90 µm |
| Crosslinked | Yes |
| Agarose % | 6% |
| Linear Recommended Flow Rate | <30 cm/h |
| Fractionation (mw) Globular Proteins Da | 1x10^4 - 4x10^6 |
| Exclusion Limit Da | >4x10^6 |
| pH Stability Working Range | 3-13 |
| ph stability cleaning in place (cip) | 2-14 |
| Chemical Stability | Stable in all solutions commonly used in gel filtration, 2M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol, 70% Ethanol and commonly used detergents |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-30˚C |
