4% BCL Agarose Bead Standard  (50-150µm)

4% BCL Agarose Bead Standard (50-150µm)

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4% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. It is a gel filtration resin commonly used for coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.

REFERENCES:

  • De Melo, Ricardo Rodrigues et al. “Cross-Linking with Polyethylenimine Confers Better Functional Characteristics to an Immobilized β-Glucosidase from Exiguobacterium Antarcticum B7.” Catalysts 9.3 (2019): n. pag. Catalysts. 
  • Siar, El Hocine et al. “Amination of Ficin Extract to Improve Its Immobilization on Glyoxyl-Agarose: Improved Stability and Activity versus Casein.” International Journal of Biological Macromolecules 133 (2019): 412–419. International Journal of Biological Macromolecules. 
  • Vilela, Adriana Ferreira Lopes, Carmen Lúcia Cardoso, and Cesar Mateo. “An Immobilized Acetylcholinesterase as Test System to Screen New Inhibitor Drugs to Treat Alzheimer’s Disease.” Sensors and Actuators, B: Chemical 278 (2019): 196–201. Sensors and Actuators, B: Chemical. 
  • Ait Braham, Sabrina et al. “Cooperativity of Covalent Attachment and Ion Exchange on Alcalase Immobilization Using Glutaraldehyde Chemistry: Enzyme Stabilization and Improved Proteolytic Activity.” Biotechnology Progress 35.2 (2019): n. pag. Biotechnology Progress. 
  • Dal Magro, L. et al. “Pectin Lyase Immobilization Using the Glutaraldehyde Chemistry Increases the Enzyme Operation Range.” Enzyme and Microbial Technology 132 (2020): n. pag. Enzyme and Microbial Technology.
  • Min, Junwei et al. “Quantitative Phase Imaging of Cells in a Flow Cytometry Arrangement Utilizing Michelson Interferometer-Based off-Axis Digital Holographic Microscopy.” Journal of Biophotonics 12.9 (2019): n. pag. Journal of Biophotonics. 

Product Name4% BCL Agarose Bead Standard (50-150µm)
Cat. No.A-1041S-X
Bead Geometry & SizeSpherical ~50-150 µm
Bead Mean Diameter d50v~90 µm
Crosslinked Yes
Agarose % 4%
Linear Recommended Flow Rate<26 cm/h
Fractionation (mw) Globular Proteins Da7x10^4 - 2x10^7
Exclusion Limit Da>2x10^7
pH Stability Working Range3-13
ph stability cleaning in place (cip)2-14
Chemical StabilityStable in all solutions commonly used in gel filtration, 2M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol, 70% Ethanol and commonly used detergents
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-30˚C
Technical Specifications View
Procedure of Use View