2% BCL Agarose Bead Standard  (50-150µm)

2% BCL Agarose Bead Standard (50-150µm)

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2% BCL Agarose Bead Standard is a crosslinked agarose resin with a bead size of 50-150 µm. It is a gel filtration resin commonly used for coupling affinity ligands. The matrix is not pre-activated so the user needs to generate groups for coupling procedures.

REFERENCES:

  • Hanumantha Rao, N. et al. “Expression of Codon Optimized Major Capsid Protein (L1) of Human Papillomavirus Type 16 and 18 in Pichia Pastoris; Purification and Characterization of the Virus-like Particles.” Vaccine 29.43 (2011): 7326–7334. Vaccine.
  • Roberts, Jeffrey N. et al. “Genital Transmission of HPV in a Mouse Model Is Potentiated by Nonoxynol-9 and Inhibited by Carrageenan.” Nature Medicine 13.7 (2007): 857–861. Nature Medicine. 
Product Name2% BCL Agarose Bead Standard (50-150µm)
Cat. No.A-1021S-X
Bead Geometry & SizeSpherical ~50-150 µm
Bead Mean Diameter d50v~90 µm
Crosslinked Yes
Agarose % 2%
Linear Recommended Flow Rate<15 cm/h
Fractionation (mw) Globular Proteins Da7x10^4 - 40x10^6
Exclusion Limit Da>40x10^6
pH Stability Working Range3-13
ph stability cleaning in place (cip)2-14
Chemical StabilityStable in all solutions commonly used in gel filtration, 2M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol, 70% Ethanol and commonly used detergents
Antimicrobial Agent 20% Ethanol
Storage Temperature 2-30˚C
Technical Specifications View
Procedure of Use View