Agarose Beads

Customer Testimonials

SPAIN

“In my lab at CIB-CSIC, we have been using (since 1996) Ni2+-IMAC for the purification of many different His-tagged proteins at large scales (10-100 mg). We are very demanding in terms of the yields and the final purity grade of our protein preps, because both of them are key factors in the structural and biochemical characterization of proteins, our main research interest. One of the major recent improvements in our daily work has been the discovery of the high-density IDA-agarose beads developed and manufactured by ABT: They match (and in some occasions, even outperform) the results previously achieved with other commercial metal-chelating supports, specially when packing of relatively large volumes (> 5 ml) of gel are required and there is a need for a mechanically robust and reliable, yet affordable, chromatographic material. Thus ABT beads have been included as essential resources in all our recent scientific achievements (1-3)"..
1. Díaz-López,T. et al. (2006) J. Mol. Biol. 364: 909-920.
2. Giraldo,R. (2007) Proc. Natl. Acad. Sci. USA 104: 17388-17393.
3. Gasset-Rosa,F. et al. (2008) Mol. Microbiol. 68: 560-572.

Department of Molecular Microbiology · Centro de Investigaciones Biológicas (CIB-CSIC) - Madrid


“To facilitate the purification of the recombinant proteins we work with, we add a His-tag at either end of the protein which allows to purify them by metal-affinity chromatography. We use IDA-agarose manufactured by ABT both as high and low density supports. The performance of the high density one is similar to that of other commercially available while the low binding gives better results when you wish to eliminate interactions of low affinity”.

Biochemistry Department - Facultad Ciencias Químicas
Univ. Complutense - 28040 Madrid


“Our Group (IQOG-CSIC) have been using different Me2+-IMAC beads (Ni, Cu, Co and Zinc) in order to purify the His-tagged proteins. In most cases the Ni2+ metal is the best way to obtain a good final purity grade, but in some cases using recombinant enzymes (Kinase, aminoglycosiltransferase) we obtained best results with Co2+ and Zn2+ as Metal. We need large volumes of IDA-agarose beads every week, so it is very important the best ratio price/quantity, for that reason we use ABT beads instead the ones manufactured by other companies”.

CSIC- Instituto de Química Orgánica General
Dpt. Química Orgánica Biológica - Juan de la cierva 3, 28006-Madrid