"We have been studying about the patho-physiological alteration of the mucins in  gastrointestinal mucosa and  we use (since 2004) ABT-agarose beads (Plain 8% Agarose Beads Standard) column (1.0×50cm) for the analysis of mucin. The void volume fraction (Fr-1) eluted with the Triton-Tris buffer can be collected as mucin. ABT-agarose beads are easy to use and reliable substance to isolate the complex macromolecules, and reasonable because they are washable and reusable. So we can recommend ABT for separating high molecular glycoconjugates".