"My group is regularly expressing and purifying recombinant proteins, mostly in E.coli, which we routinously purify using Metal Affinity Chromatography. For a couple of years, we are now using Nickel-agarose support purchased from Agarose Beads Technologies (ABT). In the applications we are using these beads (gravity flow standard purification of His-tagged proteins), the product is working reproducibly well and reliable and we have now switched to ABT beads for all our purifications".