Agarose Beads Customer Testimonials

SPAIN
“In my lab at CIB-CSIC, we have been using (since 1996) Ni2+-IMAC for the purification of many different His-tagged proteins at large scales (10-100 mg). We are very demanding in terms of the yields and the final purity grade of our protein preps, because both of them are key factors in the structural and biochemical characterization of proteins, our main research interest. One of the major recent improvements in our daily work has been the discovery of the high-density IDA-agarose beads developed and manufactured by ABT: They match (and in some occasions, even outperform) the results previously achieved with other commercial metal-chelating supports, specially when packing of relatively large volumes (> 5 ml) of gel are required and there is a need for a mechanically robust and reliable, yet affordable, chromatographic material. Thus ABT beads have been included as essential resources in all our recent scientific achievements (1-3)"..
1. Díaz-López,T. et al. (2006) J. Mol. Biol. 364: 909-920.
2. Giraldo,R. (2007) Proc. Natl. Acad. Sci. USA 104: 17388-17393.
3. Gasset-Rosa,F. et al. (2008) Mol. Microbiol. 68: 560-572.
Department of Molecular Microbiology · Centro de Investigaciones Biológicas (CIB-CSIC) - Madrid

“To facilitate the purification of the recombinant proteins we work with, we add a His-tag at either end of the protein which allows to purify them by metal-affinity chromatography. We use IDA-agarose manufactured by ABT both as high and low density supports. The performance of the high density one is similar to that of other commercially available while the low binding gives better results when you wish to eliminate interactions of low affinity”.
Biochemistry Department - Facultad Ciencias Químicas
Univ. Complutense - 28040 Madrid

“Our Group (IQOG-CSIC) have been using different Me2+-IMAC beads (Ni, Cu, Co and Zinc) in order to purify the His-tagged proteins. In most cases the Ni2+ metal is the best way to obtain a good final purity grade, but in some cases using recombinant enzymes (Kinase, aminoglycosiltransferase) we obtained best results with Co2+ and Zn2+ as Metal. We need large volumes of IDA-agarose beads every week, so it is very important the best ratio price/quantity, for that reason we use ABT beads instead the ones manufactured by other companies”.
CSIC- Instituto de Química Orgánica General
Dpt. Química Orgánica Biológica - Juan de la cierva 3, 28006-Madrid

UK
“...We have since purchased 500 ml of ABT resin and have used this to purify several proteins in our lab. We have purified all-alpha helical and all-beta sheet proteins, from 100 - 300 amino acids in size, expressed in E.coli. We find that the ABT resin performs as well as that of other leading manufacturers but costs less. This has allowed us to make a significant saving as we work on many mutant proteins and therefore use a lot of pre-charged resin”.
Dept of Chemistry - University of Cambridge
Cambridge, Cambridgeshire CB2 1EW

DENMARK
"In our research within micro-biotechnology we often have a need for customized solutions that suits our technology platform. Agarose Bead Technologies has provided us with excellent customized beads in a short time, which was essential for the successfulness of our research project. Thus, we have no problems recommending ABT when it comes to product quality and delivery".
Department of Micro and Nanotechnology - Technical University of Denmark, Build. 345 East Dk-2800 - Lyngby, Denmark

JAPAN
"We have been studying about the patho-physiological alteration of the mucins in  gastrointestinal mucosa and  we use (since 2004) ABT-agarose beads (Plain 8% Agarose Beads Standard) column (1.0×50cm) for the analysis of mucin. The void volume fraction (Fr-1) eluted with the Triton-Tris buffer can be collected as mucin. ABT-agarose beads are easy to use and reliable substance to isolate the complex macromolecules, and reasonable because they are washable and reusable. So we can recommend ABT for separating high molecular glycoconjugates".
Regulation Biochemistry Dept.- Kitasato Univ. Graduate School of Medical Sciences · Sagamihara, Kanagawa, Japan

GERMANY
"My group is regularly expressing and purifying recombinant proteins, mostly in E.coli, which we routinously purify using Metal Affinity Chromatography. For a couple of years, we are now using Nickel-agarose support purchased from Agarose Beads Technologies (ABT). In the applications we are using these beads (gravity flow standard purification of His-tagged proteins), the product is working reproducibly well and reliable and we have now switched to ABT beads for all our purifications".
Zentrum für Molekulare Biologie der Universität Heidelberg,
ZMBH Im Neuenheimer Feld 282 - D-69120 Heidelberg Germany